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Seroconversion of type B to O erythrocytes using recombinant Glycine max α‐D‐galactosidase
Author(s) -
Vosnidou Nancy C.,
Johnson Scott A.,
Mitra Moon Moon,
Wells Donald C.,
Li Chun Qin,
Evans Maria L.,
Harmata Michael A.,
Walker John C.,
Smith Daniel S.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203682
Subject(s) - erythrocyte fragility , hemolysis , red blood cell , recombinant dna , chemistry , biochemistry , epitope , glycine , red cell , hemoglobin , blood type (non human) , microbiology and biotechnology , antigen , abo blood group system , biology , immunology , medicine , amino acid , gene
Recombinant α‐D‐galactosidase (rGal) from soybean (Glycine max) hydrolyzed the immunodominant α‐D‐galactose residue from the B epitope of red blood cells. This converted type B erythrocytes to type O which are “universally” transfusable. Type B red blood cells were obtained from four different donors and enzymatically converted. Cell function parameters, including red cell indices, pH, methemoglobin, carboxyhemoglobin, osmotic fragility, hemolysis, 2,3‐diphosphoglycerate, cholinesterase, ATP, and antigen typing of treated ceils were compared to controls. These pilot studies indicate that rGal could have potential biotechnical application in the production of universally transfusable red blood cells.