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Effects of eicosapentaenoic acid and its 15‐hydroperoxy and 15‐hydroxy derivatives on glucosamine synthetase activity in rabbit gastric mucosa
Author(s) -
Fujita Tadashi,
Sakuma Satoru,
Yamamoto Naho,
Fujimoto Yohko
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203662
Subject(s) - eicosapentaenoic acid , glucosamine , chemistry , enzyme , biochemistry , adduct , gastric mucosa , mucus , hyaluronic acid , fatty acid , stomach , biology , organic chemistry , polyunsaturated fatty acid , ecology , genetics
The effects of eicosapentaenoic acid and its 15‐hydroperoxy and 15‐hydroxy adducts on the activity of glucosamine synthetase, the rate‐limiting enzyme of mucus synthesis, in rabbit gastric antral mucosa were examined. 15‐Hydroperoxy‐eicosapentaenoic acid inhibited the glucosamine synthetase activity at concentrations of 10, 20 and 50 μM. The effect was concentration‐dependent, and the concentration required for 50% inhibition was approximately 20 μM. Eicosapentaenoic acid and its 15‐hydroxy adduct had no significant effect on the enzyme activity at the same concentration range. The experiment utilizing Fe2+ revealed that the inhibitory effect of 15‐hydroperoxyeicosapentaenoic acid on the glucosamine synthetase activity is not due to hydroxy radical which is expected to be formed from the hydroperoxy adduct. These results suggest that 15‐hydroperoxyeicosapentaenoic acid has the potential to reduce the synthesis of gastric mucus by inhibiting the glucosamine synthetase activity.