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The β subunit of chloroplast ATP synthase (CF0CF1‐ATPase) is phosphorylated by casein kinase II
Author(s) -
Kanekatsu Motoki,
Saito Hitoshi,
Motohashi Ken,
Hisabori Toru
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203602
Subject(s) - chloroplast , protein subunit , atp synthase , atpase , phosphorylation , biochemistry , casein kinase 2 , g alpha subunit , protein kinase a , microbiology and biotechnology , casein kinase 2, alpha 1 , biology , enzyme , chemistry , casein kinase 1 , cyclin dependent kinase 2 , gene
We studied a phosphate acceptor for casein kinase II (CK‐II) in chloroplasts, and found a 56 kDa protein (p56) as an acceptor, which was partially purified from the stroma of spinach chloroplasts. The N‐terminal amino acid sequence of p56 was identical with that of the β subunit of chloroplast ATP synthase (CF0CF1‐ATPase). In addition, the recombinant β subunit of CF1 was phosphorylated when the subunit was incubated with CK‐II. These results suggest that the β subunit of CF1 is a substrate protein of CK‐II in the chloroplast.