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EPC‐K1 attenuates peroxynitrite‐induced apoptosis in cerebellar granule cells
Author(s) -
Wei Taotao,
Chen Chang,
Zhao Baolu,
Xin Wenjuan,
Mori Akitane
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203592
Subject(s) - peroxynitrite , apoptosis , chemistry , cerebellum , microbiology and biotechnology , granule (geology) , biophysics , neuroscience , biochemistry , biology , superoxide , enzyme , paleontology
Apoptosis induced by peroxynitrite in cultured cerebellar granule cells was confirmed morphologically by chromatin condensation and biochemically by DNA laddering. A 30 min exposure to peroxynitrite (10 μM) initiated oxidative stress, which caused the formation of thiobarbituric acid‐reactive substances (TBARS) and the alteration of cell membrane fluidity. Peroxynitrite treatment also caused ATP decrease and thus activated the apoptotic program. Pre‐treating cells with antioxidant EPC‐K1 (L‐ascorbic acid 2‐[3,4‐dihydro‐2,5,7,8‐tetramethyl‐2‐(4,8,12‐trimethyltridecyl)‐2H‐1‐benzopyran‐6‐yl‐hydrogen phosphate] potassium salt), a new water‐soluble derivative of vitamin C and vitamin E, attenuated oxidative injury and prevents cells from apoptosis. The results suggest that EPC‐K1 might be used as a potential therapeutic agent for diseases associated with NO/ONOO‐‐mediated neuronal injury.