Premium
Heat stress affects the glucocorticoid receptor interaction with heat shock protein Hsp70 in the rat liver
Author(s) -
Cvoro Aleksandra,
Dundjerski Jadranka,
Trajkovic Divna,
Matic Gordana
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203562
Subject(s) - hsp70 , glucocorticoid receptor , heat shock protein , cytosol , glucocorticoid , medicine , endocrinology , receptor , cytoplasm , basal (medicine) , hsp90 , biology , chemistry , microbiology and biotechnology , biochemistry , enzyme , insulin , gene
The association of glucocorticoid hormones receptor (GR) with heat shock protein Hsp70 in the liver cytosol of rats exposed to 41°C whole body hyperthermic stress was examined by quantitative immunoblotting of the two proteins within immunopurified untransformed GR multiprotein complexes. The presence of Hsp70 in the rat liver GR heterocomplexes was confirmed, and 2‐fold increase in the Hsp70 relative to the steroid binding protein content within the complexes was recorded 2 and 12 h after the stress. This increase exceeded the stress‐induced elevation in the total cytoplasmic Hsp70 level, but could not be seen 24 h after the stress, when cytoplasmic Hsp70 returned to basal level. The results suggest that hyperthermic stress alters the composition of the rat liver untransformed GR heterocomplexes increasing the Hsp70 share.