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Gene encoding a human testis sertoli cell component related to LIM domain protein
Author(s) -
Yang J. X.,
Miao S. Y.,
Wu Y. W.,
Zhang Z.,
Zong S. D.,
Wang L. F.,
Koide S. S.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203502
Subject(s) - lim domain , sertoli cell , gene , complementary dna , microbiology and biotechnology , northern blot , biology , open reading frame , coding region , akt1s1 , lrp1b , amino acid , peptide sequence , genetics , hspa2 , spermatogenesis , transcription factor , endocrinology , zinc finger
The cDNA (HED‐2) encoding a 20 kDa protein found in mammalian epididymal fluid was isolated from a human testis expression library. It is composed of 1908 bp, containing a reading frame of 1479 bp, coding a polypeptide consisting of 493 amino acids, and assigned the accession number: U 15158 by GenBank (Biochem. Mol. Biol. Int. 34, 1131‐1136, 1994). HED‐2 has 99% identity with the zyxin gene in amino acid sequence, a component of cell junction matrix and a member of the LIM domain protein family. Northern blot analysis of RNAs prepared from various human tissues showed that the HED‐2 gene was expressed in all tissues analyzed. Sertoli cells of human testis expressed the gene as determined by an in situ hybridization method. The present study shows that the HED‐2 gene is a member of the LIM domain protein family.