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Monoclonal antibodies directed against lipopolysaccharide of legionella pneumophila serogroup 1
Author(s) -
Baranov A. M.,
Vetchinin S. S.,
Baranova E. V.,
Biketov S. F.,
Svetoch E. A.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203392
Subject(s) - monoclonal antibody , microbiology and biotechnology , legionella pneumophila , lipopolysaccharide , antigen , antibody , biology , virology , legionella , bacterial outer membrane , spleen , virulence , strain (injury) , serotype , bacteria , escherichia coli , immunology , gene , biochemistry , genetics , anatomy
The monoclonal antibodies (MAbs) against lipopolysaccharide of virulent strain of Legionella pneumophila serogroup 1 were produced. Three most productive hybrid clones (5F4, 5F10 and 2C9) were selected from fusions of mouse myeloma cells with spleen cells from BALB/c mice, immunized with bacterial outer membrane antigens. All generated clones were IgG‐secreting. The MAbs had narrow strain specificity mad showed no cross‐reactions with other unrelated bacterial species. These antibodies were tested in sandwich ELISA. The results suggest that the MAbs could be used for diagnostic purposes.