Premium
Spin trapping for nitric oxide produced in LPS‐treated mouse using various new dithiocarbamate iron complexes having substituted proline and serine moiety
Author(s) -
Nakagawa Hidehiko,
Ikota Nobuo,
Ozawa Toshihiko,
Masumizu Toshiki,
Kohno Masahiro
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203352
Subject(s) - nitric oxide , adduct , chemistry , spin trapping , dithiocarbamate , moiety , medicinal chemistry , nuclear chemistry , inorganic chemistry , stereochemistry , organic chemistry
Four dithiocarbamate derivatives of 4‐substituted L‐proline and N‐methyl‐L‐serine were synthesized, and their iron complexes were prepared in Tris‐HCl buffer solution. These complexes were used as spin trapping reagents for nitric oxide in ESR spectrometry, and compared with each other in regard to their spin trapping properties in vivo. When the synthesized complexes were injected to lipopolysaccharide‐treated mice intravenously, the nitric oxide adducts were detected both in the liver and in the blood except N‐dithiocarboxy‐4‐(methoxymethyl)oxy‐L‐proline iron complex, whose nitric oxide adduct was detected mostly in the blood. When the exogenous nitric oxide adduct of this complex was injected, it was not detected in the liver, too. It is considered that this complex can trap nitric oxide in the blood by excluding the accumulation of the nitric oxide adduct in the liver.