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A human B cell line AF10 expressing hIL‐17
Author(s) -
Zhou Limei,
Peng Shanyun,
Duan Jubao,
Zhou Jing,
Wang Lihong,
Wang Jiaxi
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203332
Subject(s) - cell culture , biology , microbiology and biotechnology , cell , fibroblast , genetics
AF10, a human B cell line, is a mycoplasma‐free variant cloned from the human IgE myeloma cell line U266. Total RNA isolated from the AF10 cells was used as template for RT‐PCR, and a specific product of about 411bp corresponding to the coding region of hIL‐17 lack of a leading sequence obtained. The sequence of the RT‐PCR product is the same to that reported in the literature. The expressed rhIL‐17 in E. coli can induce 10‐ to 15‐fold increase of IL‐6 secretion by mouse fibroblast 3T3 cells. It is for the first time until now that hIL‐17 messager has been detected in B cell. There may exist some potential relationship between hIL‐17 and myeloma cells.