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Effect of seasonal acclimatization on the expresion of the carp transcription factor Pit‐1
Author(s) -
Kausel Gudrun,
Vera María Inés,
Figueroa Jaime,
Hernández Jaime,
Martin Rody San,
Molina Alfredo,
Chavez Viviana Marcela,
Muller Marc,
Martial Joseph,
Krauskopf Manuel
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203242
Subject(s) - carp , acclimatization , biology , in situ hybridization , gene expression , pars intermedia , gene , fish <actinopterygii> , anatomy , andrology , zoology , microbiology and biotechnology , endocrinology , pituitary gland , botany , genetics , fishery , hormone , medicine
We isolated a clone comprising four exons of the carp Pit‐1 gene. Using synthetic oligonucleotide probes derived from the carp Pit‐1 sequence Pit‐1 expression was assessed by in situ hybridization in pituitary sections from summer‐ and winter‐acclimatized carp. Semiquantitative analyses of the hybridization signals revealed a significant higher Pit‐1 expression in the proximal pars distalis (PPD) and pars intermedia (PI) of the pituitary glands from summer‐acclimatized carp, compared to the winter‐acclimatized fish. In both adaptive states, relative to the PPD and PI, only a basal Pit‐1 expression was detected in the rostral pars distalis. Thus, during seasonal acclimatization of an eurythermal fish, Pit‐1 seems to be involved in the mechanisms that underlie the compensatory response.