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Effects of D‐glucose and starvation upon the cyclic ADP‐ribose content of rat pancreatic islets
Author(s) -
Scruel O.,
Wada T.,
Kontani K.,
Sener A.,
Katada T.,
Malaisse W. J.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203202
Subject(s) - starvation , pancreatic islets , islet , chemistry , endocrinology , medicine , biochemistry , microbiology and biotechnology , insulin , biology
Rat pancreatic islets were found to display a much lower content of immunoreactive CD38 and a much lower ADP‐ribosyl cyclase activity than rat spleen or brain. Cyclic ADP‐ribose was also measured by a radioimmunological procedure in rat pancreatic islets. In fed rats, the cyclic ADP‐ribose content appeared higher after isolation of the islets in the presence of 2.8 mM D‐glucose rather than in the absence of the hexose, progressively increased during incubation of the islets for 5‐60 min at 37°C, but failed to be affected by the concentration of D‐glucose (zero to 20.0 mM) in the incubation medium. In rats fasted for 24 hours, the cyclic ADP‐ribose islet content also increased during incubation, but again failed to be affected by the concentration of D‐glucose in the incubation medium. Although these findings indicate that the islet cyclic ADP‐ribose content is influenced by nutritional and environmental factors, they do not support the view that the insulinotropic aciton of D‐glucose involves major change in the islet cell content of the cyclic nucleotide.