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Activation of cAMP synthesis in rat brain cortical membranes by rubidium and cesium ions
Author(s) -
Rosenthal Katri,
Lember Jaanus,
Karelson Ello,
Järv Jaak
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203152
Subject(s) - rubidium , adenylate kinase , forskolin , cyclase , chemistry , cortex (anatomy) , membrane , enzyme , metal , biophysics , potassium , biochemistry , biology , receptor , neuroscience , organic chemistry
Rubidium and cesium chlorides accelerated cAMP synthesis in rat brain cortex membranes, while other alkali metal chlorides had no influence on the rate of this process. The effect was dose‐dependent and yielded above 2‐fold activation of adenylate cyclase. It has been shown that Rb+ and Cs+ influenced directly the catalytic subunit of the enzyme and did not substitute Mg2+ in formation of the metal‐ATP complex in this reaction. The stimulatory effect of Rb+ was additive to the activation of adenylate cyclase by the half‐maximal (0.31‐μM) as well as by the saturating (10 μM) forskolin concentrations, pointing to the fact that these effectors activate different isoforms of the enzyme in rat brain cortex.