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Cloning and expression analysis of a cDNA coding for a dexamethasone‐inducible cytochrome P450 in Xenopus laevis liver
Author(s) -
Ohi Hiroaki,
Sugata Eiji,
Fujita Yoshiaki,
Saito Hiromi,
Saguchi Kenichi,
Murayama Nobuhiro,
Higuchi Shigesada
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203092
Subject(s) - complementary dna , xenopus , biology , subfamily , microbiology and biotechnology , cdna library , coding region , cytochrome p450 , cloning (programming) , gene , rapid amplification of cdna ends , clone (java method) , gene expression , molecular cloning , genetics , biochemistry , enzyme , computer science , programming language
Abstract We previously purified a cytochrome P450 (P450) from liver microsomes of adult female Xenopus laevis. In this study, we screened a cDNA library of Xenopus liver to isolate the cDNA clone coding for this P450. The 5′‐end of the resultant cDNA was truncated at the N‐terminal region and extended by method of rapid amplification of eDNA end to give the complete coding sequence. Amino acid sequence showed this clone to be 36% to 55% identical to members of the CYP2 family and less than 31% identical to members of other gene famines, and to belong to the CYP2Q subfamily. This gene is expressed constitutively in the livers of adult male and female frogs, and is significantly induced by dexamethasone administration.