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DNA‐binding properties of the overexpressed recombinant estrogen receptor α
Author(s) -
Ikeda Mitsunori,
Tsuji Norio,
Kikukawa Kyoko,
Asahar Yoshie,
Nakashima Aya,
Minatogawa Yohsuke
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203072
Subject(s) - estrogen receptor , receptor , recombinant dna , hormone response element , chemistry , nuclear receptor , microbiology and biotechnology , estrogen related receptor gamma , estrogen related receptor alpha , estrogen receptor beta , transformation (genetics) , biophysics , biochemistry , biology , gene , transcription factor , genetics , cancer , breast cancer
Estrogen receptor α was overexpressed in COS‐7 cells by transformation of an expression vector with the full length open reading frame of the receptor α. The nuclear estradiol‐receptor complex and the soluble receptor from the COS‐7 cells were cross‐linked with an estrogen response element (ERE), which was substituted with 5‐bromo‐2′‐deoxyuridine (BrdUVRE), as the receptor dimers by UV irradiation. In gel retardation analysis, the specific bindings of both nuclear and soluble receptors to ERE were decreased with increasing of KCl concentration compared with 0.1 M KCl. The ionic interactions of both receptors with ERE are thought to be similar.