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Binding of naturally occurring anti‐DNA antibodies to estradiol
Author(s) -
Ali Asif
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202892
Subject(s) - autoantibody , dna , immunoassay , antibody , microbiology and biotechnology , enzyme , recombinant dna , beta (programming language) , chemistry , biology , immunology , biochemistry , gene , computer science , programming language
Binding of naturally occurring human anti‐DNA autoantibodies to β‐estradiol and native DNA has been investigated. Sera from seven SLE patients were tested for their reactivity with β‐estradiol and native calf thymus DNA. Solid Phase enzyme immunoassay was performed using both colorigenic and fluorogenic substrates. The results show enhanced binding of human anti‐ DNA autoantibodies to β‐estradiol as compared to native DNA. This was further substantiated by increased binding of affinity purified SLE IgG to β‐estradiol. These results suggest a role for female sex hormone in the etiopathogenesis of SLE and are significant vis‐a‐vis female predominance of SLE.