Premium
Immunological studies on DNA‐lysine photoadduct
Author(s) -
Islam Najmul,
Ali Rashid
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202842
Subject(s) - lysine , dna , chemistry , biology , computational biology , genetics , microbiology and biotechnology , biochemistry , amino acid
Calf thymus native DNA was covalently linked with lysine by irradiation with 254 nm light. Thermal melting studies indicated a decrease of 16°C in the Tm of DNA‐lysine photoadduct over native DNA. The decrease in Tm was dependent of irradiation dose. The photoadduct was found to be a potent immunogen in experimental animal. Thymine‐lysine photoconjugate in DNA‐lysine photo‐monoadduct appears to be the major immunodominant portion. A strong recognition of DNA‐lysine photoadduct was observed with anti‐DNA autoantibodies found in the sera of patients with SLE. Thymine‐lysine conjugate in DNA‐lysine photo‐monoadduct appears to provide an immunodominant epitope(s) for SLE autoantibody recognition. The results suggests for the possible involvement of DNA‐lysine photoadduct or similar modified structure(s) as a potential trigger for anti‐DNA autoantibody production.