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Induction of apoptosis by polyunsaturated fatty acids and its relationship to fatty acid inhibition of carnitine palmitoyltransferase I activity in Hep2 cells
Author(s) -
Colquhoun Alison
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202702
Subject(s) - polyunsaturated fatty acid , docosahexaenoic acid , apoptosis , arachidonic acid , eicosapentaenoic acid , fatty acid , biochemistry , linolenic acid , chemistry , degree of unsaturation , linoleic acid , biology , enzyme , chromatography
The effects of fatty acids on the induction of apoptosis were studied over a 24 hour period in Hep2 human larynx tumour cells. While oleic and linoleic acids had little effect on the apoptotic index, the polyunsaturated fatty acids α‐linolenie, γ‐linolenic, arachidonic, eicosapentaenoic and docosahexaenoic acids all induced apoptosis, beginning at ∼6 hours after fatty acid exposure. By 24 hours after exposure, the apoptotic index had reached as much as 19% in the presence of docosahexaenoic or α‐iinolenic acid. The correlation between degree of unsaturation of the 18‐carbon fatty acids and the apoptotic index was r2=0.45, 0.89, 0.84 and 0.98 at 6, 8, 12 and 24 hours, respectively. The correlation between mitochondrial carnitine palmitoyltransferase I (CPT I) activity, 6 hours after exposure, and the apoptotic index was r2=0.842 and 0.798 at 12 and 24 hours, respectively. The inhibition of CPT I and subsequent fatty acid oxidation by polyunsaturated fatty acids leads to a significant increase in apoptosis, suggesting that CPT I may be involved in the processes of programmed cell death in Hep2 human turnout cells.