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The synthesis of HSPs in sugar beet suspension culture cells under hyperthermia exhibits differential sensitivity to calcium
Author(s) -
Kuznetsov Vladimir V.,
Andreev Igor M.,
Trofimova Marina S.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202642
Subject(s) - heat shock protein , calcium , extracellular , microbiology and biotechnology , protein biosynthesis , heat shock , hyperthermia , sugar beet , biochemistry , calcium in biology , cell culture , shock (circulatory) , biology , chemistry , biophysics , intracellular , medicine , paleontology , genetics , organic chemistry , gene , horticulture
Experiments aimed at testing the effect of Ca2+ on heat shock‐induced changes in protein synthesis of cultured sugar beet cells were performed. Heat shock inhibits the synthesis of non‐heat shock proteins (non‐HSPs) and promotes the synthesis of a set of HSPs. Extracellular Ca2+ appeared to be strictly required for the synthesis of non‐HSPs. Calcium was found to differentially exert its effect on the HSP synthesis: calcium induced (96 and 76 kDa), stimulated (94, 67, 58, 52, 32, 30, 26 and 22 kDa) or did not influence (82, 17 kDa) the de novo production of various HSPs. Cell injury increased if the cells were exposed to high temperature in a Ca2+‐deficient medium. Calcium supplement to Ca2+‐depleted cells resulted in the recovery of HSP synthesis and reduced cell injury by heat shock.