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Luminol luminescence induced by oxidants in antioxidant‐deficient yeasts Saccharomyces cerevisiae
Author(s) -
Jakubowski Witold,
Bartosz Grzegorz,
Ertel Danuta,
Bilinski Tomasz,
Kędziora Józef
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202562
Subject(s) - chemiluminescence , menadione , hydrogen peroxide , luminol , superoxide dismutase , catalase , chemistry , antioxidant , yeast , reactive oxygen species , biochemistry , glutathione , superoxide , peroxide , saccharomyces cerevisiae , photochemistry , enzyme , chromatography , organic chemistry
Luminal chemiluminescence induced in the presence of yeast cells and yeast cell homogenates was significantly induced by exogenous oxidants (hydrogen peroxide and menadione), tert‐Butyl hydroperoxide did not stimulate chemiluminescence by itself but augmented menadione‐induced chemiluminescence. Comparison of yeast strains deficient in catalase, superoxide dismutase or glutathione showed that only glutathione‐deficient strains showed elevated chemiluminescence in this system. These results support the idea that more reactive species than hydrogen peroxide and superoxide are critical in the induction of luminal chemiluminescence.