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Soluble guanylate cyclase in Saccharomyces cerevisiae
Author(s) -
Kuo W. N.,
Kanadia R. N.,
Mcnabb M.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202492
Subject(s) - saccharomyces cerevisiae , guanylate cyclase , chemistry , gucy1a3 , biochemistry , gucy1b3 , guanylate cyclase 2c , yeast , enzyme
Abstract After removing nonspecific immunoreactivities from crude extract by immunoaffinity chromatography, an immunoreactive‐band at 40kDa of soluble guanylate cyclase (SGC) from Saccharomyces cerevisiae was detected by Western blot using rabbit anti‐β1 subunit of SGC. Cyclic GMP level and SGC activity was measured by ELISA. Immunoprecipitated yeast SGC was activated by sodium nitroprusside, whereas inhibited by 1H‐(1,2,4) oxadiazolo(4,3‐A)quinoxalin‐1‐one. Increased cyclic GMP level was also noted when intact yeast cells were incubated with s‐nitrosoglutathione, a NO donor. The result implies that NO can be utilized intracellularly and extracellularly. Moreover, the presence of SGC suggests the significance of NO/cyclic GMP signaling in unicellular eukaryotes.