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A method for uni‐directional reconstitution of human erythrocyte glucose transporter
Author(s) -
Xiao L.,
Xie W. S.,
Yang F. Y.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202312
Subject(s) - glucose transporter , liposome , transporter , chemistry , phospholipid , biophysics , chromatography , permeability (electromagnetism) , biochemistry , membrane , biology , gene , insulin , endocrinology
The glucose transporter of human erythrocytes (Glutl) was reconstituted into soybean phospholipid liposomes by a method of direct incorporation using the nonionic detergent n‐octyl β‐D‐glucopyranoside. The reconstituted proteoliposomes were proved to be intact and low ionic permeability. Freeze‐fracture electron microscopy study showed that the diameter of the proteoliposomes was about 150±50 nm and the protein was randomly distributed. The kinetic parameters of the reconstituted transporter were: Km=16.23 mmol/L, Vmax=34.48 nmol/sec.mg protein. Furthermore, about 90% of the glucose transporter in the reconstituted proteoliposomes were orientated inside‐out. Until now it is a more efficient method for uni‐directional reconstitution of Glut 1 with good reproducibility and higher transport activity.