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Effects of short‐ and long‐term exposure to linoleic acid hydroperoxide on cytosolic calcium ion level of human aortic intimal smooth muscle cells In vitro
Author(s) -
Arima Nobuyuki,
Sasaguri Yasuyuki,
Yagi Kunio
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202272
Subject(s) - calcium , cytosol , calcium channel blocker , chemistry , aorta , calcium channel , channel blocker , in vitro , medicine , biochemistry , endocrinology , biophysics , biology , enzyme , organic chemistry
When cultured intimal smooth muscle cells from human aorta were exposed for a short period of time (25 sec) to linoleic acid hydroperoxide (100 nmol/ml), influx of calcium ions into the cytosol of these cells was provoked, and a temporal increase in cytosolic calcium ion level was observed. Two calcium channel blockers inhibited this influx. Long‐term exposure (4 min) of these cells to the hydroperoxide also provoked the influx of calcium ions, which resulted in a longer time of the calcium ion increase. In the latter case, however, the calcium channel blocker inhibited the initial influx, but then the influx started and continued even in the presence of the blocker. Such difference in exposure time‐dependent effects should be taken into account in considering pathological roles of lipid hydroperoxides.