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Vaccinia virus‐induced changes in cytokine‐regulated acute phase plasma protein synthesis by hepatoma cells
Author(s) -
Rokita H.,
Branicki W.,
Wronska D.,
Borysiewicz L. K.,
Koj A.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202162
Subject(s) - vaccinia , cytokine , virus , virology , acute phase protein , biology , immunology , chemistry , inflammation , recombinant dna , biochemistry , gene
Abstract Human hepatoma cell line, HepG2, has been infected with vaccinia virus and synthesis of plasma proteins was determined by etectroimmunoassay and corresponding mRNA's measured by Northem blotting. The inhibitory effect of the virus was dose‐ and time‐ dependent. Electrophoretic mobility shift assay revealed a decrease in C/EBP binding activities in nuclear extracts isolated from the infected hepatoma cells. Supershift analysis of the C/EBP isoforms showed α and β subunit involvement in DNA binding. The treatment of the cells with interleukin‐1, interleukin‐6, and dexamethasone at the initital stage of infection appears to delay the virally induced inhibition of host cell protein synthesis. Thus, possible “protective” role of the acute phase cytokines in viral infection is proposed.