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V1a receptor mRNA expression in mouse early proximal tubule (S1) cell line is regulated by protein kinase A
Author(s) -
Takeda Michio,
Kobayashi Mami,
Shirato Isao,
Endou Hitoshi
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202022
Subject(s) - protein kinase c , forskolin , microbiology and biotechnology , liver receptor homolog 1 , gene expression , biology , cell culture , chemistry , receptor , signal transduction , transcription factor , gene , biochemistry , nuclear receptor , genetics
The purpose of this study was to investigate the regulation of V1a receptor gene expression in mouse early proximal tubule (S1) cell line (NF‐5). NF‐5 cells showed arginine vasopressin (AVP)‐induced increase in cytosolic free calcium concentration mediated by V1a receptor. Polymerase chain reaction coupled with reverse transcription analysis revealed that forskolin, but not phorbol‐12‐myristate‐13‐acetate stimulated V1a receptor mRNA expression in NF‐5 cells. Our data suggest that V1a receptor gene expression is regulated by protein kinase A in proximal tubule cell line, and the cell line might be a useful model for the further studies of the function and the regulation of the activity and expression of the V1a receptor in the proximal tubule.