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Fatty acid‐mediated uncoupling of potato tuber mitochondria
Author(s) -
Saviani Elzira E.,
Martins Ione S.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201882
Subject(s) - mitochondrion , uncoupling protein , uncoupling agents , biochemistry , respiration , biology , gtp' , membrane potential , fatty acid , chemistry , enzyme , botany , brown adipose tissue , adipose tissue
The present work examined whether the ATP/ADP carrier, other than the plant uncoupling mitochondrial protein, participates in free fatty acid‐mediated uncoupling of potato tuber mitochondria. The basal respiration rate of succinate‐energized mitochondria was stimulated by a low concentration of palmitate (20μM). This uncoupling was reversed by 10μM carboxyatractyloside and by the subsequent addition of 0.1% bovine serum albumin. The decrease in membrane potential caused by palmitate was suppressed by carboxyatractyloside (1μM) and, to a lesser degree, by bongkrekate (20μM). GTP could also reversed this decrease via a carboxyatractyloside‐independent mechanism. These results indicate that the ATP/ADP carrier, along with the plant uncoupling mitochondrial protein, participates in the protonophoric action of palmitate in potato tuber mitochondria.