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Role of naturally occuring autoantibodies in senescence of normal and ATP depleted goat erythrocytes
Author(s) -
Anwar Adil,
Saleemuddin Mohammed
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201852
Subject(s) - autoantibody , senescence , in vitro , peroxidase , antibody , gamma globulin , microbiology and biotechnology , biology , chemistry , biochemistry , immunology , andrology , enzyme , medicine
The possible role of autoantibodies in the senescence of goat erythrocytes has been investigated. For this purpose goat gammaglobulin was purified to homogenity and antigoat gammaglobulin was raised in rabbits. Using the peroxidase labelled antigoat gammaglobulin it was possible to detect the presence of auto anti band‐3 antibodies in goat sera. The goat erythrocytes were aged in vitro by ATP‐depletion, which resulted in appearence of a 52,000 mol. wt. polypeptide. The in vitro aged goat erythrocyte membrane bound substantial amounts of auto anti band‐3 antibodies as visualized by immumoblots.