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Establishment of a mouse clonal early proximal tubule cell line and outer medullary collecting duct cells expressing P2 purinoceptors
Author(s) -
Takeda Michio,
Kobayashi Mami,
Endou Hitoshi
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201702
Subject(s) - medullary cavity , microbiology and biotechnology , proximal tubule , duct (anatomy) , cell culture , chemistry , anatomy , biology , endocrinology , kidney , genetics
The purpose of this study was to establish tubule cells expressing P2 purinoceptors. Use of the polymerase chain reaction coupled with reverse transcription showed that mouse clonal early proximal tubule (S1) cell line (NF‐5) and outer medullary collecting tubule (OMCT) cells expressed mRNA for P2X4, P2Y1 and P2Y2 purinoceptors. ATP and its analogues induced a dose‐dependent increase in cytosolic free calcium concentration ([Ca2+i] in both NF‐5 and OMCT cells. ATP enhanced [3H]‐thymidine uptake by both NF‐5 and OMCT cells in a dose‐dependent manner. In conclusion, NF‐5 and OMCT cells can be used in the further analysis of the function and regulation of activity and expression of P2 purinoceptors in the renal tubule.