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Esterification of D‐mannoheptulose confers to the heptose inhibitory action on D‐glucose metabolism in parotid cells
Author(s) -
Malaisse Willy J.,
Kadiata Marcel M.,
Scruel Olivier,
Sener Abdullah
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201662
Subject(s) - heptose , inhibitory postsynaptic potential , metabolism , chemistry , carbohydrate metabolism , d glucose , biochemistry , action (physics) , endocrinology , medicine , biology , mutant , gene , physics , quantum mechanics
D‐mannoheptutose, but not its hexaacetate ester, inhibits, in a competitive manner, D‐glucose phosphorylation by either purified beef heart hexokinase or crude parotid gland homogenates. Yet, D‐mannoheptulose hexaacetate, but neither the unesterified heptose nor acetate or its methyl ester, inhibits D‐[5‐3H]glucose utilization and D‐[U‐14C]glucose conversion to 14CO2 and 14C‐labelled acidic metabolites aud anaino acids in intact isolated parotid cells. It is proposed, therefore, that D‐mannoheptulose hexaacetate crosses efficiently the plasma membrane of parotid cells and, after intracellular hydrolysis, allows inhibition of D‐glucose phosphorylation by the unesterified heptose. The ester of D‐mannoheptulose could thus represent a useful tool to inhibit hexose phosphorylation and interfere with cell growth in cells otherwise resistant to the heptose.