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Saccharide‐assisted delivery of cytotoxic liposomes to human malignant cells
Author(s) -
Vodovozova Elena L.,
Gayenko Galina P.,
Razinkov Vladimir I.,
Korchagina Elena Yu.,
Bovin Nikolai V.,
Molotkovsky Julian G.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201582
Subject(s) - liposome , in vitro , chemistry , cytotoxic t cell , cytotoxicity , fluorescein , drug delivery , lectin , microbiology and biotechnology , biochemistry , fluorescence , biology , physics , organic chemistry , quantum mechanics
The overexpression of lectins by malignant cells was applied for in vitro targeting of liposomes equipped with a saccharide vector and loaded in the lipid phase with a lipid derivative of anticancer agent sarcotysine. The lectin specificity of human leukemia HL‐60 and human lung adenocarcinoma ACL cells was revealed by tests with fluorescein‐labeled sugar probes. With the help of fluorescent lipid dye it was shown that active saccharide ligands increased the level of the vectored liposome binding to malignant cells by 50‐80% as compared to liposomes without vector or with inactive one. The degree of liposome/cell membrane fusion was monitored fluorometrically and was shown to be complete and independent of the vectors. The targeted drug‐loaded liposomes had the cytotoxic activity 2‐4 times higher as compared to the vector‐free ones.