Premium
Short‐term effect of dexamethasone on fatty acid and cholesterol synthesis in isolated rat hepatocytes
Author(s) -
Giudetti Anna Maria,
Gi Gabriele V.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201542
Subject(s) - dexamethasone , chemistry , fatty acid , cholesterol , term (time) , medicine , endocrinology , biochemistry , biology , physics , quantum mechanics
The short‐term effect of dexamethasone, a synthetic glucocorticoid, on both fatty acid and cholesterol synthesis has been investigated in rat hepatocyte cultures. Within 4h following hormone addition to the cultures, a noticeable stimulation of labelled acetate incorporation into fatty acids was observed. Similar behaviour was noticed when [3H]H20 was used as an independent index of the lipogenic activity. In the same cultures, however, cholesterol synthesis from both [ 14 C]acetate or [3H]H20 was significantly reduced by dexamethasone addition. In these conditions, no significative variation of cholesterol synthesis starting from labelled mevalonate was observed.