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Lithium regulation of protein phosphorylation in rat cerebral cortex slices in vitro
Author(s) -
Singh Tryambak Deo,
Shankar Raj
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201522
Subject(s) - phosphorylation , ouabain , cerebral cortex , sodium , chemistry , protein phosphorylation , in vitro , lithium (medication) , incubation , biophysics , phosphate , cortex (anatomy) , biochemistry , microbiology and biotechnology , protein kinase a , biology , endocrinology , neuroscience , organic chemistry
Abstract The aim of the present study was to evaluate the direct effect of Li+ and Na+‐Li+ exchange on protein phosphorylation in rat cerebral cortex slices incubated in Krebs Ringer medium. When Na+ concentration was varied in the incubation medium, either by replacement with Li+ or sucrose, a variable effect on [32P] phosphate incorporation into proteins was observed. Protein phosphorylation in cerebral cortex slices was very low in the absence of Na+, and some dependence of phosphorylating system of neural tissue on extra cellular concentration of Na+ was evident. Lithium was not able to replace sodium as far as protein phosphorylation in cortical slices is concerned. Ouabain was more effective in a Li+ containing medium in inhibiting protein phosphorylation, presumably due to improper functioning of the sodium pump.