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Different effects of cell‐permeable ceramide analogs on platelet activation
Author(s) -
Hashizume Tsutomu,
Kageura Tadashi,
Sato Takashi
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201512
Subject(s) - ceramide , lipid signaling , sphingomyelin , chemistry , platelet , arachidonic acid , microbiology and biotechnology , thrombin , platelet activation , biochemistry , biology , membrane , apoptosis , immunology , receptor , enzyme
The effects of cell‐permeable ceramide analogs on platelet responses induced by agonists were investigated. When washed rabbit platelets were pretreated with ceramide and then stimulated with thrombin or U46619, C2‐ceramide (N‐acetylsphingosine) dose‐dependently inhibited the aggregation and arachidonic acid liberation, whereas C6‐ceramide (N‐hexanoylsphingosine) and C8‐ceramide (N‐octanoylsphingosine) enhanced these responses. Furthermore, C6‐ceramide, but not C2‐ceramide, enhanced the increase in and prevented the progressive decrease in cytoplasmic free Ca2+ concentration induced by U46619. On the other hand, treatment with sphingomyelinase potentiated the aggregation in response to U46619. These results indicate that the effects of cell‐permeable ceramide analogs on platelet activation vary with N‐acyl chain length.