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Induction of buccal mucosal apoptosis with chronic alcohol ingestion
Author(s) -
Slomiany B. L.,
Piotrowski J.,
Piotrowski E.,
Slomiany A.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201392
Subject(s) - apoptosis , buccal swab , dna fragmentation , buccal administration , ingestion , tumor necrosis factor alpha , ethanol , necrosis , buccal mucosa , alcohol , chemistry , biology , medicine , microbiology and biotechnology , programmed cell death , biochemistry , dentistry , oral cavity
In this study we investigated buccal mucosal cells' apoptosis and the expression of regulatory cytokines, tumor necrosis factor‐α (TNF‐α) and inetrleukin‐4(IL‐4) with chronic ethanol ingestion. The buccal mucosa of rats maintained for 23 days on alcohol‐containing and control liquid diet was assessed for IL‐4 and TNF‐α content, and the extent of epithelial cells apoptosis. While the expression of TNF‐α in alcohol diet group showed a significant increase (1.9‐fold) over that of the controls, less apparent differences between the two groups were observed in the content of IL‐4 (141.8±28.2 vs. 119.8±7.3 pg/mg protein). The DNA fragmentation assays revealed that alcohol diet group also exhibited a 3.5‐fold enhancement in buccal mucosal cells apoptosis. Moreover, the apoptotic index showed positive correlation (r=0.53) with the extent of induced changes in TNF‐α. These results demonstrate that ethanol‐induced buccal mucosal cells apoptosis is triggered by the enhancement in TNF‐α expression.