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Transition protein 1 from boar late spermatid nuclei having DNA‐melting activity is a dimeric protein
Author(s) -
Akama Kuniko,
Sato Hiroki,
Hasegawa Shigeki,
Shimada Ichio,
Nakano Minoru
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201332
Subject(s) - dimer , spermatid , chemistry , intermolecular force , dna , crystallography , biophysics , biochemistry , biology , molecule , organic chemistry , sperm , botany
Polyacrylamide gel electrophoretic behavior of boar transition protein 1, TP1, under dissociating and non‐dissociating buffer conditions, and titration of fluorescently labeled TP1 with increasing amounts of TP1 showed that TP1 formed a dimer without intermolecular disulfide bond. TP1 dimer with intermolecular disulfide bond had similar DNA‐melting activity to TP1, but was not detected in extracts from boar late spermatid nuclei. These results suggest that TP1 dimer without intermolecular disulfide bond induces local destabilization of DNA in the late spermatid nuclei.