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Is the glutathione S‐conjugate pump a flippase?
Author(s) -
Sokal Adam,
Pułaski Łukasz,
Rychlik Błazej,
Fortuniak Anna,
Bartosz Grzegorz
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201102
Subject(s) - glutathione , conjugate , chemistry , flippase , biophysics , membrane , quenching (fluorescence) , biochemistry , fluorescence , biology , enzyme , phosphatidylserine , phospholipid , mathematical analysis , physics , mathematics , quantum mechanics
Abstract A hypothesis of the flippase nature of the glutathione S‐conjugate transport is presented. Experimental premises for this hypothesis include interaction of glutathione S‐conjugates with the membrane, as demonstrated by their effects on membrane fluidity, quenching of 1‐(4‐trimethylammoniumphenyl)‐6‐phenyl‐1,3,5‐hexatriene fluorescence and induction of echinocytosis by 2,4‐dinitrophenyl‐S‐glutathione (DNP‐SG). This hypothesis can rationalize, i. a., observations of the enhancement of DNP‐SG transport by butanol and stimulation of erythrocyte membrane Mg2+‐ATPase activity by albumin‐coupled DNP‐SG.