Premium
Reverse transcription‐polymerase chain reaction (RT‐PCR) for direct detection of cucumber mosaic virus (CMV) in gladiolus
Author(s) -
Raj S. K.,
Saxena Sangeeta,
Hallan Vipin,
Singh B. P.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201092
Subject(s) - gladiolus , polymerase chain reaction , biology , cucumber mosaic virus , complementary dna , reverse transcription polymerase chain reaction , virology , reverse transcriptase , microbiology and biotechnology , real time polymerase chain reaction , gene , virus , genome , plant virus , messenger rna , genetics , botany
A diagnostic method based on Reverse Transcription ‐ Polymerase Chain Reaction (RT‐PCR) was performed for detection of CMV genome directly in crude extracts of both healthy and infected gladiolus tissue using primers from the conserved sequences of CMV RNA‐3. RT‐PCR resulted in the amplification of 540 bp long fragment of CMV coat protein gene (CMV‐CP), as expected in most of the plant parts of infected gladiolus samples. Positive signals in Southern hybridization of amplified fragments with cloned CMV‐CP cDNA probe also confirmed the presence of CMV genome in gladiolus.