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Expression of the cDNA and purification of P0 ribosomal protein from Lupinus luteus
Author(s) -
Mikolajczyk Katarzyna,
Barciszewski Jan
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201072
Subject(s) - ribosomal protein , complementary dna , ribosomal rna , biology , eukaryotic small ribosomal subunit , eukaryotic large ribosomal subunit , cdna library , microbiology and biotechnology , protein subunit , 28s ribosomal rna , gene , ribosome , 18s ribosomal rna , genetics , rna
Abstract Eukaryotic ribosomal protein P0 is localized on the large ribosomal subunit at the base of the specific “stalk” structure, close to the central protuberance. Recently we have obtained a clone coding for P0 ribosomal protein from L.luteus (yellow lupin) cDNA library. Here we present its expression in E.coli cells and purification of the target protein.