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Prolactin‐induced expression of TATA‐less cyclin D3 gene is mediated by Sp1 and AP2
Author(s) -
Yang Miao,
Nomura Hideki,
Hu Ying,
Kaneko Satoshi,
Kaneko Hiroshi,
Tanaka Minoru,
Nakashima Kunio
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201052
Subject(s) - enhancer , microbiology and biotechnology , transfection , tata box , gene , oligonucleotide , electrophoretic mobility shift assay , promoter , biology , prolactin receptor , gene expression , regulatory sequence , luciferase , chemistry , prolactin , genetics , biochemistry , hormone
The TATA‐less cyclin D3 gene is stimulated by prolactin (PRL) in rat Nb2 T‐lymphocytes. DNA mobility shift assay and luciferase transfection analysis revealed that the gene has a potential promoter region, two enhancer domanins and a silencer region. The promoter region possessed three Sp1 sites and two AP2 sites, and both enhancer regions bad one AP2 binding site each. Analysis using oligonucleotide probes and purified AP2 protein has suggested that the expression of rat cyclin D3 gene is promoted by Sp1 and AP2 factors.