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Identification of a transcript predicting an alternative form of sulfated glycoprotein‐2 (clusterin) in rat tissues
Author(s) -
Izawa Masao
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201012
Subject(s) - clusterin , complementary dna , biology , glycoprotein , seminal vesicle , messenger rna , cdna library , microbiology and biotechnology , androgen , apoptosis , gene , prostate , biochemistry , genetics , hormone , cancer
As a clue to understand the role of androgen in rat seminal vesicle, we have isolated several cDNA clones of androgen‐responsive mRNAs. During further characterizations of these cDNAs were in progress, a distinct cDNA clone designated p116 was isolated. The sequence study suggested the expression of a transcript predictding an alternative form of Sulfated Glycoprotein‐2 (SGP‐2)/clusterin. RT‐PCR demonstrated the androgen‐dependent expression of p116 transcript in the seminal vesicles. The transcript was also detectable in the ventral prostate, the liver and the thymus. Since SGP‐2 has been suggested as a classical molecular marker of apoptosis, p116 transcript newly identified in the present study might provide a useful probe to further understand the roles of SGP‐2 and its related proteins during the course of apoptotic process not only in male accessory sex organs, but also in many types of cells undergoing apoptosis.