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Biochemical properties of hemagglutinins in the mollusk Pomacea flagellata
Author(s) -
ArreguínEspinosa Roberto,
ArreguínLozano Barbarín
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700205071
Subject(s) - glycoprotein , hapten , agglutinin , isoelectric point , isoelectric focusing , biochemistry , galactose , chemistry , divalent , galactosides , biology , antibody , lectin , enzyme , immunology , organic chemistry
Extracts of the Mollusk Pomacea flagellata were found to contain two agglutinins (PFA‐I and PFA‐II) which reacted with erythrocytes from human and several animal species. The hemagglutinating activity was stable between pH 4.0 and pH 10.0, and was independent of divalent cations. The active components were purified and found to be glycoproteins having Mr of 30,000. The isoelectric points were estimated to be 4.7 and 4.9 for PFA‐I and PFA‐II respectively. Hapten inhibition assays indicated that the agglutinin and its isoforms are specific for β‐galactosides as well as for galactose containing glycoproteins.