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Anti‐ROS‐DNA monoclonal antibody as molecular probe for oxidative DNA damage
Author(s) -
Ashok Badithe T.,
Ahmad Jabeen,
Ali Rashid,
Qadri Ayub
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700205051
Subject(s) - guanine , monoclonal antibody , dna , nucleic acid , dna damage , thymine , microbiology and biotechnology , epitope , chemistry , oxidative phosphorylation , dna oxidation , oxidative stress , biochemistry , antibody , biology , nucleotide , immunology , gene
Modification of 400 bp (approximate size) calf thymus DNA with OH radical resulted in lowered Tm, modification of thymine (58.3%), guanine (20%) and single strand breaks. Monoclonal antibodies (mAb) generated against ROS‐DNA were of IgG1 subclass. The mAb showed strong binding to ROS‐DNA and ROS‐modified bases and polymers, in particular, of thymine. The mAb, therefore, preferentially recognizes ROS‐modified epitopes on nucleic acids. Distinct binding to DNA isolated from aged, but not from normal humans by the monoclonal antibdy was observed. The antibody effectively recognized oxidative lesions in DNA from cancer patients. These studies demonstrate the potential application of the mAb as an immunochemical probe to detect oxidative DNA lesions.