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Vitamin D receptor in IMR‐90 human fibroblasts and antiproliferative effect of 1,25‐dihydroxyvitamin D3
Author(s) -
Stio M.,
Celli A.,
Lunghi B.,
Raugei G.,
Modesti A.,
Treves C.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700205011
Subject(s) - calcitriol receptor , receptor , chemistry , vitamin d and neurology , cancer research , medicine , endocrinology , microbiology and biotechnology , biology , biochemistry
This study examines the effect of 1,25‐dihydroxyvitamin D3 [1,25(OH)2D3], serum or forskolin on the proliferation of IMR‐90 fetal lung fibroblasts and demonstrates, for the first time, the presence of 1,25(OH)2D3 receptor (VDR) in this cell line. In quiescent, subconfluent cultures neither the treatment with 100 nM 1,25(OH)2D3 nor that with 50 μM forskolin influenced proliferation, while a significant increase was observed after incubation of the cells with 10% fetal bovine serum. Either cell number, determined on growing IMR‐90 human fibroblasts after 48 or 72 h incubation with 100 nM 1,25(OH)2D3 or [3H]thymidine incorporation (24, 48 or 72 h incubation) significantly decreased, while protein content per cell increased. Northern blot analysis revealed the expression of the VDR gene, the VDR mRNA bands being prominent in 1,25(OH)2D3, serum or forskolin treated fibroblasts. VDR mRNA levels slightly decreased, when growing fibroblasts were exposed to 100 nM 1,25(OH)2D3 for 48 or 72 h.