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Identification of chlorella T‐1 ingredient which enhances DMPO‐OH adduct formation in fenton reaction
Author(s) -
Ali M. Aktar,
Oykawa Kikuo,
Ohwada Minoru,
Konishi Tetsuya
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204601
Subject(s) - chemistry , adduct , chlorella , ingredient , methanol , lactic acid , chromatography , carbon fixation , radical , algae , photosynthesis , nuclear chemistry , organic chemistry , food science , biochemistry , bacteria , botany , genetics , biology
Chlorella T‐1 is a micro algae which carries out highly active photosynthetic carbon fixation. For searching the ·OH scavenging molecules in this strain, the methanol extract of T‐1 was fractionated by RP‐HPLC and the fractions were analyzed for DMPO‐OH formation by ESR after Fenton reaction. In the course of study, a single chlorella T‐1 ingredient was found to enhance the formation of DMPO‐OH rather than scavenge in the Fenton system. The ·OH enhancing chlorella component was further purified by chromatography and finally identified as lactic acid by NMR and LC‐MS. From the analysis of the reaction conditions for DMPO‐OH, it was found that lactic acid did not serve as direct source of ·OH but accelerated Fenton reaction.