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Isolation of cDNA encoding full‐length rat (Rattus norvegicus) poly(ADP‐ribose) polymerase
Author(s) -
Beneke Sascha,
Meyer Ralph,
Bürkle Alexander
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204571
Subject(s) - polymerase , poly adp ribose polymerase , microbiology and biotechnology , biology , dna polymerase , biochemistry , dna clamp , complementary dna , dna , polymerase chain reaction , gene , reverse transcriptase
Poly(ADP‐ribose) polymerase (EC 2.4.2.30) is a nuclear enzyme which binds to DNA breaks and then catalyzes the covalent modification of acceptor proteins with poly(ADP‐ribose). Poly(ADP‐ribose) polymerase activity contributes to the recovery of proliferating cells from DNA damage and to the maintenance of genomic stability, which may be mediated by effects on chromatin structure, DNA base‐excision repair and cell cycle regulation. We established the complete eDNA sequence of rat poly(ADP‐ribose) polymerase by RT‐PCR and direct sequencing of amplification products and compared it with that of other mammalian species. The amino acid sequence homology is strikingly high. The best conserved regions are the known functional modules ofpoly(ADP‐ribose) polymerase.