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Cloning and functional analysis of rat cyclin D2 promoter: multiple prolactin‐responsive elements
Author(s) -
Yang Miao,
Hosokawa Yoshitaka,
Hu Ying,
Kaneko Satoshi,
Kaneko Hiroshi,
Tanaka Minoru,
Nakashima Kunio
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204561
Subject(s) - cloning (programming) , tata box , cyclin d2 , cyclin a , microbiology and biotechnology , gene , cyclin , cyclin e , cyclin d1 , cyclin d , prolactin , transcription factor , transcription (linguistics) , biology , cell cycle , chemistry , promoter , gene expression , genetics , biochemistry , hormone , linguistics , philosophy , computer science , programming language
Structure and function of the promoter region of rat cyclin D2 gene were investigated by cloning, sequence analysis and DNA mobility shift assay using nuclear extracts of prolactin‐stimulated Nb2 cells. The results show that the expression of rat cyclin D2 gene is not dependent on the TATA‐box but is stimulated by multiple transcription factors induced by prolactin at an immediate‐early stage of the cell cycle.