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Inhibition of interleukin‐6‐induced human immunodeficiency virus type 1 expression by anti‐GP130 monoclonal antibody
Author(s) -
Okamoto Mika,
Yasukawa Kiyoshi,
Katsuura Kimio,
Baba Masanori
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204541
Subject(s) - glycoprotein 130 , monoclonal antibody , biology , virology , cell culture , monocyte , interleukin 19 , virus , microbiology and biotechnology , flow cytometry , interleukin , cytokine , antibody , immunology , interleukin 6 , genetics , interleukin 5
Interleukin 6 (IL‐6) has been reported to upregulate the expression of human immuno‐deficiency virus type 1 (HIV‐1) in infected monocyte/macrophages. Since IL‐6 signal is transduced through the membrane glycoprotein gp130, we investigated the inhibitory effect of anti‐gp130 monoclonal antibody (mAb) on IL‐6‐induced viral expression in monocytic cell lines latently infected with HIV‐I. IL‐6 significantly induced the expression of HIV‐1 in U1 cells, whereas it had no effect in OM‐10.1 cells. Flow cytometric analysis revealed that U1 but not OM‐10.1 cells expressed gp130 on their surface. The anti‐gp130 mAb could inhibit IL‐6‐induced HIV‐1 expression in U1 cells in a dose dependent fashion. These results suggest that blocking the gp130 signal transduction may have therapeutic potential for the treatment of HIV‐1 infection.