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Studies on growth‐promoting action of insulin: mitogenic activity of insulin and its analogues in mouse mammary tumor cells
Author(s) -
Shi Min,
Feng YouMin
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204511
Subject(s) - insulin , medicine , endocrinology , insulin receptor , biological activity , mammary tumor , biology , thymidine , chemistry , biochemistry , in vitro , insulin resistance , cancer , breast cancer
Growth‐promoting action of insulin and its analogues was studied in the mouse mammary tumor cell line GR2H6 by measuring 3H‐thymidine incorporation. The mitogenic activity of the analogues obtained by shortening of C‐terminus of insulin B chain decreased with stepwise removal of terminal residues, and [A3Leu]insulin was approximately 1000 times less potent than insulin. However, the despentapeptide(B26‐B30) insulin(DPI) showed a strikingly higher mitogenic activity at high concentrations; the detected maximal effect of DPI was 136% more than that of insulin. These results demonstrate that the C‐terminal area of the B chain and the N‐terminal α‐helix of the A chain are important for the mitogenic activity of insulin. A region corresponding to the growth‐promoting function of insulin and a model of DPI action are proposed and discussed.