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Cloning of the human cDNA sequence encoding the NADH: Ubiquinone oxidoreductase MLRQ subunit
Author(s) -
Kim Jae Wha,
Lee Younghee,
Kang Ho Bum,
Choe Yong Kyung,
Chung Tae Wha,
Chang Sung Yeoul,
Lee Kwang Soo,
Choe In Seong
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204471
Subject(s) - complementary dna , cdna library , biology , microbiology and biotechnology , open reading frame , homology (biology) , peptide sequence , oxidoreductase , protein subunit , molecular cloning , nucleic acid sequence , cloning (programming) , amino acid , genomic library , biochemistry , gene , enzyme , computer science , programming language
A cDNA clone encoding human NADH:ubiquinone oxidoreductase (complex I of mitochondrial respiratory chain) MLRQ subunit was isolated from human fetal liver cDNA library. The clone contained an open reading frame of 246 bp which predicted a protein comprising 81 amino acids with a calculated molecular weight of 9,370 Da. The deduced amino acid sequence exhibited 95% homology (88% identity and 7% favored substitution) to that of bovine MLRQ subunit. Northern analysis revealed that the cDNA clone hybridized with a 0.7 kb mRNA species which was present in all tissues examined. The expression level of the 0.7 kb mRNA in heart, skeletal muscle, and brain was higher than in other organs. Human MLRQ cDNA could cross‐hybridize with the genomic DNAs from various species.