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Binding characteristics of sle anti‐DNA autoantibodies to modified DNA analogue
Author(s) -
Arjumand Sadia,
Ali Asif
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204451
Subject(s) - dna , chemistry , denaturation (fissile materials) , antibody , fluorescence , molecule , microbiology and biotechnology , furocoumarins , titration , biochemistry , recombinant dna , fluorescence spectroscopy , biology , photochemistry , organic chemistry , immunology , physics , quantum mechanics , nuclear chemistry , gene
Native calf thymus DNA was modified with furoconmarins and reactive oxygen species (ROS). The modifications were probed by UV & Fluorescence spectroscopy, thermal denaturation and hydroxyapatite chromatography. In CD spectroscopy changes in the ellipticity of the DNA molecule were observed as a result of modification. The binding specificity of naturally occurring anti‐DNA antibodies with modified DNA molecules was assessed by ELISA and quantitative precipitin titration. The affinity ofanti‐DNA antibodies for modified conformers was found to be quite high.