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Purification of recombinant human precursor acid α‐glucosidase
Author(s) -
Van Hove Johan L. K.,
Yang Helen W.,
Oliver Letealia M.,
Pennybacker Mark F.,
Chen YuanTsong
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204421
Subject(s) - recombinant dna , affinity chromatography , concanavalin a , chromatography , hydrophilic interaction chromatography , chemistry , enzyme , ion chromatography , yield (engineering) , in vivo , biochemistry , high performance liquid chromatography , in vitro , biology , materials science , microbiology and biotechnology , metallurgy , gene
Large quantities of recombinant acid α‐glucosidase are needed for in viva experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A affinity chromatography, hydrophobic interaction chromatography, affinity chromatography on Superdex, and anion exchange chromatography. The new method is amenable to scale up, and has increased speed, and improved reproducibility with similar high yield and purification efficiency when compared to previous methods.